Isolation and culture of slime molds

West Patel Nagar New Delhi: Morphological and reproductive characterization will also be conducted on the isolated fungi. However, the fungal isolates from shrimp exoskeleton, cockroaches and rabbit dung were successful.

Isolation and Culture of Slime Molds Essay Sample

Set up for moist chamber, B. The authors would also like to thank their professors, Sir Thomas Edison dela Cruz and Sir Mike Valdez, for their unending guidance and patience. Besides being commonly found in lakes, streams, ponds, roadside ditches and coastal marine environments, chytrids also are present in soil.

I tried germinating the spores under a foldscope, with little success and so I tried culturing them. A slime mold in my garden mulch And so while I was reading the book, I decided to mulch my little garden for winter and lo and behold there was this yellow growth in the mulch Figure 3A.

Baiting technique is a method that allows further growth of fungal mycelia on prepared baits.

Exploring a Slime Mold-1: Detection, isolation and culturing

All of these will be stored for days under cool conditions. Preparation of RDA and PDA for pure isolates of the cultures Correct amount of potato dextrose agar was measured using the triple beam balance. All of the baits were examined under the microscope after 2 days and at daily intervals thereafter.

Observations were recorded by taking pictures seen under the microscope. The fungal isolates from the pollen grain are saprotrophic fungi that should degrade polymers. However they differ from other fungi, in producing motile, flagellated spores called zoospores, a possible remnant of their adaptation to aquatic environment, mostly freshwater and moist soils, where they are commonly found.

Fungi and Allied Microbes.

How do they deal with infections by viruses and bacteria? Fungal isolates from dungs are coprophilous fungi that degrade the waste and other nutrients in the animal dung. Acknowledgements The authors are grateful to the staffs of Botanical Garden and Microbiology laboratory of the University of Santo Tomas for help in obtaining the materials.

What molecules facilitate communication between the cells before fusion and after fusion, How is communication possible within the giant plasmodium… endless and fascinating questions, but for us with foldscopes our first goal is to see them.

Slime molds or myxomycetes and their life cycles. The cell walls of oomycetes are typically made of cellulose, not chitin, as for fungi, and they have flagellated cells, which are lacking in all but a few fungi.

The prepared media was subjected to autoclave before pouring on plates. The number of PDA plates that were prepared was according to the number of fungal zoospores observed on the cultures of shrimp exoskeleton and cockroach. RDA media was also prepared by boiling one rabbit dung per ml of distilled water.

They depend on their zoospores for dispersal and site-selection. Baits one bait for one plate were placed on the pond water-filled disposable petri dishes. The hyphae, reproductive structures and zoospores were to observe.

Culturing Slime Mold

Exploring a Slime Mold Figure 3C and D show magnifications of 3x and 10x taken with the lens-light provided with the new foldscope kit.

Moist environment will help for the growth of the fungi. On the other hand, fungal isolates from exoskeleton are chitinolytic fungi.

Fresh soil samples were collected and placed inside a disposable petri plates.Keywords:myxomycetes, slime molds, natural products, culture, fruit body, pigment, bisindole, triterpenoid Abstract: The Myxomycetes (true slime molds) are an unusual group of primitive organisms that may be assigned to one of the lowest classes of eukaryotes.

Slime Mold Cultures

Once the slime mold has grown to completely cover the top of the culture dish, hang paper towels along the insides of the larger container down into the water. The slime mold will eventually travel to the paper towel. At this point the paper towels can be removed and placed in a moist terrarium.

If culturing to grow slime mold for future use, remove the paper towel and allow it to dry at room temperature. Slime Molds There are over different types of slime molds, or myxomycetes as the scientific world calls them.

Slime molds have been difficult to classify for years do to the fact that they exhibit characteristics of both fungi and animals. Differ from cellular slime molds by having a large, multi-nucleated super-amoeba form with thousands of nuclei Are large and brightly colored Visible form is the plasmodium when individual flagellated cells swarm together and fuse.

ISOLATION, CULTIVATION, AND CONSERVATION OF SIMPLE SLIME MOLDS BY KENNETH B. RAPER Fermentation Division, Northern Regional Research Laboratory, Peoria, Illinois.

Each culture contains enough material for a class of 30 students. Helpful Ordering Hint: To ensure you order enough living materials for the number of students in your class, order 1 culture for up to 30 students, 2 cultures for 31 to 60 students, 3 cultures for 61 to 90 students, etc/5(17).

Isolation and culture of slime molds
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